Mouse S100A9 PharmaGenie ELISA Kit (SBRS1518)

Calprotectin subunit S100A9, the calcium-binding protein also known as MRP14 or calgranulin B, is abundantly expressed in neutrophils and infiltrating monocytes and dimerizes with S100A8 to form the heterodimer calprotectin, an alarmin released at sites of inflammation. Assay Genie's Mouse S100A9 PharmaGenie ELISA Kit provides a quantitative immunoassay for measuring this analyte in samples harvested from laboratory mice, supporting research into arthritis, colitis, sepsis, and other neutrophil-driven disease models.

This 96 assays format kit is supplied as a complete set with a pre-coated microplate, lyophilized standards, detection antibody, and assay buffer sufficient to run a standard curve alongside unknown samples. The procedure produces an absorbance readout on a standard microplate reader at the wavelength specified in the protocol, and the single-use strip-well plate layout lets researchers run partial plates without wasting unused wells. The kit supports ex vivo work with cell culture supernatant and matched blood draws from laboratory mice.

Working with the reagents: reconstituted standards and aliquoted detection components should be handled to limit freeze-thaw cycles, and the kit should be stored refrigerated with protection from light between uses. Application areas include kinetic profiling of inflammatory markers in murine disease models, compound screening in mouse inflammation studies, and supporting cell culture investigations of monocyte and neutrophil biology.