Mouse S100A8 ELISA Kit

This sandwich ELISA kit from Assay Genie uses a double-antibody format with colorimetric detection and is designed for the precise measurement of S100A8 levels in mouse serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay delivers a sensitivity of 0.375 ng/mL across a working range of 0.625-40 ng/mL.

S100A8 (Uniprot: P27005) is a key protein involved in inflammatory processes, with documented roles in immune response and tissue repair. It serves as a biomarker for inflammatory conditions including rheumatoid arthritis, inflammatory bowel disease, and sepsis. The kit is listed under the cell death research area and is intended for use in immunology and drug development studies. All reagents should be stored at 2-8°C for 12 months.

Included components are a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody (100X concentrate), HRP-streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X), plate sealer, and a technical manual. The protocol proceeds through reagent equilibration, primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding for 60 minutes, HRP-streptavidin incubation for 30 minutes, dark TMB color development, and final absorbance measurement at 450 nm after adding stop solution.