Mouse S-adenosylmethionine synthase isoform type-2 (Mat2a) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA is designed for the quantitative detection of mouse S-adenosylmethionine synthase isoform type-2 (Mat2a), an enzyme that catalyses the formation of S-adenosylmethionine (SAMe) from methionine and ATP. The kit uses a colorimetric ELISA detection method and is intended for research applications in cell biology.

The assay is validated for use with mouse serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with reactivity confirmed for mouse samples. Specificity covers natural and recombinant mouse S-adenosylmethionine synthase isoform type-2. According to the manufacturer's specifications, the detection range is 0.312-20ng/mL with a sensitivity of 0.178ng/mL. Linearity has been evaluated across serum (91-100%, 88-98%, 99-109%, 83-94% at 1:2, 1:4, 1:8 and 1:16 dilutions respectively), EDTA plasma (109-119%, 89-101%, 89-99%, 91-101%) and heparin plasma (86-98%, 105-115%, 104-114%, 96-106%). Reported recovery is 80% (range 80-80%) for both serum and plasma samples.

Synonyms listed for the target include methionine adenosyltransferase 2, MAT 2 and AdoMet synthase 2. The associated UniProt accession is Q3THS6, with a reported molecular weight of 43,689 Da. Reagents are stored at either -20°C or 4°C depending on the component. The product is supplied for research use only.