Mouse RUNX2 ELISA Kit

A sandwich ELISA, double antibody kit from Assay Genie designed for the precise detection of RUNX2 levels in mouse samples. The colorimetric assay accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is positioned for developmental biology research focused on bone physiology and skeletal disorders.

Quantitative performance is supported by a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Spiked recovery averaged 98% in serum (n = 5), 97% in EDTA plasma (n = 5), and 95% in heparin plasma (n = 5), with corresponding recovery ranges of 86–103%, 88–103%, and 85–105%. Linearity across 1:2, 1:4, and 1:8 dilutions was 97–101% / 86–104% / 87–103% for serum, 88–97% / 82–99% / 82–100% for EDTA plasma, and 87–98% / 80–100% / 82–100% for heparin plasma. The kit is stored at 2–8°C for 12 months.

RUNX2 is described as a vital transcription factor that regulates bone development and osteoblast differentiation, with dysregulation linked to osteoporosis and skeletal abnormalities. The associated UniProt entry is Q08775, with listed synonyms including CBFA1, AML3, Core-binding factor subunit alpha-1, PEBP2A, and runt-related transcription factor 2.