Mouse Rps14/ 40S ribosomal protein S14 ELISA Kit

Designed by Assay Genie for the quantitative measurement of 40S ribosomal protein S14 (RPS14) in mouse samples, this sandwich ELISA kit uses a double-antibody format to deliver high sensitivity and specificity for research applications. RPS14 is a vital component of the 40S ribosomal subunit, playing a crucial role in protein synthesis, and its dysregulation has been linked to various diseases including cancer, developmental disorders, and autoimmune conditions.

The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. It delivers a sensitivity of 46.875 pg/mL with a working range of 78.125–5000 pg/mL, and components are intended to be stored at 2–8°C for 12 months. Reported linearity for serum spans 85–96% at a 1:2 dilution, 82–97% at 1:4 and 81–100% at 1:8; EDTA plasma shows 85–103%, 88–101% and 87–99% at the same dilutions, while heparin plasma spans 85–101%, 83–99% and 82–98%. Recovery data indicate an average of 93% for serum (range 88–97%), 99% for EDTA plasma (range 94–103%) and 95% for heparin plasma (range 90–104%).

Detection is performed colorimetrically by measuring absorbance at 450 nm after TMB substrate development, with HRP-Streptavidin signal amplification. The kit serves as a tool for researchers investigating ribosomal protein biology and its implications in disease pathology.