Mouse Ribonucleoside-diphosphate reductase subunit M2 (Rrm2) ELISA Kit

A sandwich ELISA from Assay Genie designed for the quantitative measurement of Ribonucleoside-diphosphate reductase subunit M2 (RRM2) in mouse samples, supporting research into DNA synthesis and repair, cellular proliferation and disease mechanisms. RRM2 is described as a key enzyme in DNA synthesis and repair, contributing to cellular proliferation and maintenance, and its dysregulation has been implicated in diseases such as cancer, making it a relevant biomarker for studies of disease progression and potential therapeutic interventions.

The kit uses a sandwich ELISA format with colorimetric detection and is reactive with mouse samples. Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Specificity is directed against natural and recombinant mouse Ribonucleoside-diphosphate reductase subunit M2. The target, also known by the synonyms ribonucleotide reductase small chain and ribonucleotide reductase small subunit, corresponds to UniProt entry P11157. Functionally, it provides the precursors necessary for DNA synthesis, catalyses the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides, and inhibits Wnt signalling. The protein is localised to the cytoplasm and forms a heterodimer of a large and a small subunit. It is grouped under the research area Epigenetics.

Manufacturer-reported performance characteristics include a detection range of 0.312-20 ng/mL and a sensitivity of 0.175 ng/mL. Precision is given as an intra-assay CV of 6.4% and an inter-assay CV of 8.9%. Recovery is reported at an average of 104% (range 98-110%) for serum and 106% (range 100-112%) for plasma. Dilutional linearity data are provided for serum, EDTA plasma and heparin plasma across 1:2, 1:4, 1:8 and 1:16 dilutions. For research use only.