Mouse RHOA (Ras Homolog Gene Family, Memmber A) ELISA Kit (MOES01778)

Quantify endogenous Ras homolog gene family member A (RHOA) in mouse-derived specimens with this solid-phase immunoassay from Assay Genie, supplied as a complete kit containing reagents for 96 determinations on a pre-coated microplate. RHOA is a small GTPase that cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch that remodels the actin cytoskeleton, drives actomyosin contractility, and controls cell migration, adhesion, polarity, and proliferation. Dysregulated RHOA signaling has been implicated in tumor invasion, lymphoma biology, vascular remodeling, and fibrotic responses, making quantitative measurement valuable for oncology, immunology, cell biology, and cardiovascular research programs.

The kit accepts liquid mouse samples such as cleared tissue homogenates and is intended for research use only in laboratory workflows such as academic core facilities, pharmaceutical target validation, and biomarker discovery. Each package contains the pre-coated plate, detection reagents, and a recombinant RHOA reference standard sufficient to generate a standard curve across the assay's working range. Colorimetric readout is performed on a standard microplate reader, and absorbance values are interpreted against the supplied reference standard to calculate sample concentrations.

Researchers commonly pair this ELISA with orthogonal validation such as western blot, RT-qPCR, or RhoA GTPase pull-down assays to confirm transcriptional, total protein-level, and activation-state changes. Keep the sealed kit refrigerated upon arrival to preserve reagent activity through the stated shelf life, protect the detection antibody from light, and avoid repeated freeze-thaw cycles of diluted working solutions.