Mouse Rho guanine nucleotide exchange factor 1 (Arhgef1) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the precise and reliable detection of mouse Rho guanine nucleotide exchange factor 1 (ARHGEF1) in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. ARHGEF1 is described as a key regulator of Rho GTPases involved in cell migration, cytoskeletal organization, and cell adhesion, and its dysregulation has been linked to cancer and cardiovascular disorders.

The kit operates over a detection range of 0.312-20ng/mL with a sensitivity of 0.184ng/mL, using a colorimetric ELISA detection method. It is specific for natural and recombinant mouse ARHGEF1. Performance figures for intra-assay CV, inter-assay CV, linearity, and recovery are provided with the kit.

ARHGEF1 acts as a guanine nucleotide exchange factor (GEF) for RhoA GTPase and as a GTPase-activating protein (GAP) for GNA12 and GNA13. Activated Gα13 stimulates its RhoGEF activity through the RGS-like domain, while binding to activated GNA12 inhibits this activity. The protein mediates angiotensin-2-induced RhoA activation; isoforms 3 and 4 lack homooligomerization and display enhanced RhoGEF activity. The target is encoded by Arhgef1 (UniProt Q61210; NCBI Gene ID 16801), with a molecular weight of 109,249 Da. Known synonyms include Lbc's second cousin, Lymphoid blast crisis-like 2, Lbcl2, and Lsc. The protein localizes to the cytoplasm and membrane, with translocation to the membrane upon GNA13 or LPA stimulation. For research use only.