Mouse RF-IgM (Rheumatoid Factor IgM) ELISA Kit (MOFI01458)

The Mouse RF-IgM ELISA Kit from Assay Genie enables quantitative measurement of Rheumatoid Factor IgM autoantibodies in mouse research samples. RF-IgM is a key serological marker widely studied in models of rheumatoid arthritis, including collagen-induced arthritis (CIA) and antigen-induced arthritis (AIA), supporting longitudinal monitoring of autoimmune disease progression and therapeutic intervention studies.

The indirect biotin-based immunoassay uses IgG-coated microplate wells to capture circulating RF, with biotinylated anti-mouse IgM detection antibodies producing a chromogenic signal proportional to RF-IgM concentration. The protocol accommodates a 50 µL sample volume per well and is completed within a standard working day using the provided calibrators and reagents.

For research use only, this kit supports applications including autoimmune mechanism studies, drug efficacy screening in arthritic mouse models, biomarker validation, and comparative immunology investigations across inbred mouse strains. Reagent components should be stored refrigerated and protected from light; reconstituted standards and working solutions should be aliquoted to limit freeze-thaw cycles.