Mouse Retinal dehydrogenase 1 (Aldh1a1) ELISA Kit

A colorimetric sandwich ELISA from Assay Genie for quantitative measurement of mouse Retinal dehydrogenase 1 (ALDH1A1). Described as a powerful tool for accurate measurement of ALDH1A1 levels, the kit offers high sensitivity and specificity. ALDH1A1 is an important enzyme involved in retinoic acid biosynthesis, and dysregulation has been linked to cancer, retinal disorders, and neurological disorders.

Reported specifications include a detection range of 78-5000pg/ml, sensitivity of 39pg/mL, intra-assay CV of 6.8%, and inter-assay CV of 9.3%. Linearity (N=5) for serum spans 83-93% at 1:2 to 104-114% at 1:16; EDTA plasma spans 80-89% at 1:2 to 101-110% at 1:16; heparin plasma spans 112-121% at 1:2 to 107-115% at 1:16. Recovery averages 105% (range 99-111%) in serum and 107% (range 101-113%) in plasma. The assay is reactive with mouse and accepts serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with specificity for natural and recombinant mouse Retinal dehydrogenase 1.

ALDH1A1 can convert and oxidize retinaldehyde to retinoic acid, binds free retinal and cellular retinol-binding protein-bound retinal, and may oxidise other aldehydes in vivo. The protein has a molecular weight of 56.3 kDa, forms a homotetramer, and is localised to the cytoplasm and cytosol (UniProt P24549). Listed synonyms include ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase cytosolic, RALDH 1, Ahd-2, Ahd2, and Aldh1. The listed research area is cancer. The kit is for research use only.