Mouse Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit

Regulator of G-protein Signaling 6 belongs to the R7 subfamily of RGS proteins and forms constitutive complexes with the atypical G-protein subunit Gβ5, functioning as a GTPase-activating protein that accelerates GTP hydrolysis on Gα subunits of heterotrimeric G proteins. By dampening GPCR signaling duration, RGS6 modulates cardiac stress responses, neuronal excitability, and tumor cell behavior. This immunoassay kit from Assay Genie enables quantitative protein measurement in mouse-derived research specimens using a complete reagent set that includes a pre-coated plate, calibrated standards, detection antibody, wash buffer, and substrate solution.

The kit is intended for research use only and is validated on mouse tissue homogenates, with applicability to additional specimen types within the validated performance range. Reconstituted standards and working detection antibody should be stored refrigerated, while unused strips remain stable in the foil pouch with desiccant until the printed expiration date. Each plate supports multiple standard-curve replicates alongside unknown samples processed in single-use wells.

Investigators studying Gβ5-RGS6 complex assembly, cardiac GPCR desensitization, doxorubicin-induced cardiotoxicity, or RGS6 tumor suppressor activity in melanoma and breast cancer mouse models can integrate this assay into expression profiling panels alongside western blot and immunohistochemistry workflows. Absorbance readout on a standard microplate reader provides quantitative protein concentration data normalized to total input.

Assay Genie supplies technical support documentation and lot-specific certificates of analysis to assist with intra- and inter-assay precision assessment in longitudinal mouse studies.