Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit

The Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit from Assay Genie enables quantitative measurement of murine CCL5, a key CC-chemokine released by activated T lymphocytes, platelets, and endothelial cells that orchestrates leukocyte trafficking during inflammation. This immunoassay supports research into viral pathogenesis, airway hyperresponsiveness, transplant rejection, and autoimmune disease models, where CCL5 concentrations in clarified tissue homogenates and cell culture supernatants serve as biomarkers of immune activation. The protocol uses a pre-coated microplate, sequential incubation with a biotinylated detection antibody and streptavidin-HRP conjugate, and a chromogenic substrate read on a standard microplate reader. Reconstituted standards and detection antibody vials are stored refrigerated between uses, and the substrate solution should be protected from light and prepared fresh. Each Assay Genie kit ships with pre-measured reagents, a wash buffer concentrate, and step-by-step instructions sufficient to quantify chemokine concentrations across a standard curve.