Mouse RANK ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the quantitative detection of RANK (Receptor Activator of Nuclear Factor Kappa B) in mouse samples. The assay is reactive with mouse and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The kit delivers a sensitivity of 37.5 pg/mL across a working range of 62.5-4000 pg/mL. Storage is recommended at 2-8°C for 12 months. Reported validation includes linearity across serum (83-100%, 91-98%, 84-95% at 1:2, 1:4, 1:8 dilutions; n=5), EDTA plasma (80-93%, 88-99%, 86-103%; n=5), and heparin plasma (86-105%, 88-100%, 81-93%; n=5), alongside recovery values of 95-102% (average 97%) for serum, 92-99% (average 94%) for EDTA plasma, and 85-103% (average 98%) for heparin plasma (n=5 each).

RANK is a key regulator of bone metabolism and immune function, involved in bone remodeling, osteoclast differentiation, and immune response, making it a relevant biomarker for research into osteoporosis, arthritis, and autoimmune diseases. The procedure involves sequential incubations with biotin-labeled detection antibody, HRP-Streptavidin, and TMB substrate, with absorbance read at 450 nm.