Mouse R-Spondin 1 (RSPO1) ELISA Kit

RSPO1, the secreted activator of canonical Wnt/β-catenin signaling, can be quantitatively measured in mouse tissue homogenates, cell lysates, and related samples using this antibody-pair microplate immunoassay from Assay Genie. The kit supplies 96 assays per box and targets the R-Spondin 1 protein, which is studied in intestinal stem-cell niche maintenance, gonadal development, and keratinocyte biology.

Working with a standard microplate-based absorbance readout, researchers load prepared samples alongside a dilution series of provided recombinant standards to derive a standard curve, then interpolate unknowns against it. Results are typically reported as protein concentration in ng/mL or pg/mL, and the format accommodates the small sample volumes typical of lysate-based workflows. Each kit ships with a pre-coated microplate, detection antibody, recombinant standards, buffer reagents, and substrate; absorbance is recorded on a standard plate reader at the indicated endpoint.

For sample handling, prepare cell lysates in standard lysis buffer and tissue homogenates freshly on ice before loading; aliquot samples to limit freeze-thaw cycles, and store kit components at the temperatures indicated in the product insert upon receipt. Equilibrate reagents to room temperature before running. The kit is configured for research use only, supporting applications such as Wnt pathway analysis, intestinal stem cell research, and mouse model characterization where RSPO1 expression is a variable of interest.