Mouse Pyruvate Kinase, Muscle (PKM2) ELISA Kit

Assay Genie's PKM2 ELISA Kit supports quantitative measurement of pyruvate kinase muscle isoform 2 in mouse-derived samples for metabolism and oncology research. PKM2 catalyzes the final rate-limiting step of glycolysis, converting phosphoenolpyruvate to pyruvate with concurrent ATP generation, and is widely investigated in studies of the Warburg effect, tumor metabolism, and immune cell activation. The kit is supplied in a 96 assays format configured for in vitro research use, enabling investigators to compare PKM2 abundance across treatment cohorts in mouse models.

Typical applications include tracking PKM2 expression under hypoxic culture conditions, monitoring metabolic reprogramming during oncogenic transformation, and assessing responses to pharmacological inhibitors in cell culture or tissue homogenates. Each kit contains pre-coated microplates along with the standard and detection reagents required to generate a calibration curve for interpolating unknown sample concentrations. Researchers working on cancer metabolism, T-cell biology, or metabolic disease pathways can integrate this assay into longitudinal studies to characterize how PKM2 levels shift with experimental perturbation.