Mouse Pyruvate Kinase, Liver And RBC (PK) ELISA Kit

Pyruvate kinase (PK) is a pivotal regulatory enzyme in the final step of glycolysis, catalyzing the transfer of a phosphate group from phosphoenolpyruvate to ADP to generate ATP and pyruvate. This quantitative immunoassay from Assay Genie is configured for the mouse liver (L-PK) and red blood cell (M-PK) isoforms and supports quantification in serum, tissue homogenates, cell lysates, and cell culture supernatants. Investigators studying erythrocyte energy metabolism, hepatocyte glycolytic control, PK deficiency, or hemolytic anemia mouse models use this kit to measure PK levels in biological specimens. The package contains pre-coated microplate wells, lyophilized reference standards, a biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent — sufficient reagents for 96 determinations. Each plate is shipped in foil-sealed wells to preserve reagent stability, and the workflow is compatible with standard plate-reader instrumentation commonly found in metabolism and hematology laboratories. The assay is intended for research use only and is not validated for diagnostic procedures.