Mouse Protein phosphatase 1 regulatory subunit 1A (Ppp1r1a) ELISA Kit

Designed for the quantitative measurement of protein phosphatase 1 regulatory subunit 1A (PPP1R1A) in mouse samples, this Sandwich ELISA Kit from Assay Genie provides a highly sensitive and specific colorimetric assay for accurate detection of PPP1R1A levels. The kit is validated for use with serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids, and detects both natural and recombinant mouse PPP1R1A.

Performance characteristics include a detection range of 0.156 - 10 ng/mL and a sensitivity of 0.09 ng/mL. Precision is reported as an intra-assay CV of 6.4% and an inter-assay CV of 8.9%. Recovery in serum averages 85% (range 80 - 91%) and in plasma averages 87% (range 81 - 93%).

PPP1R1A acts as an inhibitor of protein-phosphatase 1 and is implicated in hormonal control of glycogen metabolism; cAMP-elevating hormones increase I-1 activity, and a rise in intracellular calcium leads to inactivation by calcineurin (PP2B). The subunit interacts with PPP1R15A and corresponds to UniProt entry Q9ERT9 (molecular weight 18,718 Da; NCBI Gene ID: 58200). The kit is supplied in a solid phase format, with storage at -20°C and shipping at +4°C. For research use only.