Mouse Protein kinase C alpha type (Prkca) ELISA Kit

Designed for the quantitative measurement of mouse Protein kinase C alpha type (PRKCA), this sandwich ELISA from Assay Genie is reactive with mouse samples and validated for use with serum, plasma, and cell culture supernatants, with broader compatibility for tissue homogenates and other biological fluids. The kit specifically recognises natural and recombinant mouse Protein kinase C alpha type.

The assay delivers a detection range of 31.2-2000pg/ml with a sensitivity of 15.6pg/mL. Precision is supported by an intra-assay CV of 7.9% and an inter-assay CV of 9.4%. Linearity has been confirmed in serum, EDTA plasma, and heparin plasma at 1:2, 1:4, 1:8, and 1:16 dilutions, with serum recovery averaging 85% (range 80-91) and plasma recovery averaging 87% (range 81-93).

PRKCA is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase (UniProt: P20444) that participates in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration, adhesion, cardiac hypertrophy, angiogenesis, platelet function, and inflammation. The protein is localised to the cytoplasm, cell membrane, mitochondrial membrane, and nucleus, and is a research focus in cancer. The kit is for research use only.