Mouse Protein delta homolog 1 (Dlk1) ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative detection of mouse Protein delta homolog 1 (Dlk1), also listed under synonyms including Adipocyte differentiation inhibitor protein, Preadipocyte factor 1, Pref-1, DLK-1, Dlk, Pref1, and Scp-1 [C0, S1]. The kit is intended for use with mouse-derived samples including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and is read using a colorimetric ELISA detection method [C0, S1].

The assay reports a detection range of 0.312–20ng/mL and a sensitivity of 0.167ng/mL, with an intra-assay CV of 6.9% and an inter-assay CV of 9.0% [C0, S1]. Specificity is reported for natural and recombinant mouse Protein delta homolog 1 [S1]. Reported linearity across serial dilutions (N=5) is 98–107%, 89–100%, 102–111% and 81–91% for serum; 92–102%, 91–101%, 84–94% and 100–111% for EDTA plasma; and 89–100%, 105–115%, 108–116% and 90–100% for heparin plasma [S1]. Recovery values average 92% (range 86–98%) for serum and 94% (range 88–100%) for plasma [S1].

DLK1 is described as a protein that may have a role in neuroendocrine differentiation and that inhibits adipocyte differentiation, and it is classified within the cardiovascular research area [S1]. The target is annotated as a single-pass type I membrane protein that functions as a monomer, with UniProt accession Q09163 [S1]. The kit is supplied for research use only [S1].