Mouse Prostatic acid phosphatase (Acpp) ELISA Kit

Designed for the quantitative detection of mouse prostatic acid phosphatase in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, this Sandwich ELISA from Assay Genie uses a colorimetric detection method and is reactive with mouse samples. The reagents supplied are intended for research use only.

Validated performance specifications include a detection range of 0.312–20 ng/mL and a sensitivity of 0.156 ng/mL. Reported intra-assay CV is 4.9% and inter-assay CV is 7.1%. Linearity has been assessed in serum, EDTA plasma and heparin plasma (n=5) at dilutions of 1:2, 1:4, 1:8 and 1:16, spanning 106–118% in serum, 86–120% in EDTA plasma and 83–117% in heparin plasma. Recovery averaged 95% in serum (range 89–101%) and 97% in plasma (range 91–103%). Specificity is reported for natural and recombinant mouse prostatic acid phosphatase.

ACPP is described as a non-specific tyrosine phosphatase that dephosphorylates a range of substrates under acidic conditions (pH 4–6) and exhibits lipid phosphatase activity, inactivating lysophosphatidic acid in seminal plasma. The protein is documented to play a key role in prostate development and function, as well as in the regulation of apoptosis and cell differentiation. Isoform 2 additionally possesses ecto-5'-nucleotidase activity in dorsal root ganglion neurons, generating adenosine from AMP that acts as a pain suppressor. The functional unit is a homodimer, with dimer formation required for phosphatase activity, and the marker is associated with prostate cancer and related research areas.