Mouse Propionyl-CoA carboxylase beta chain, mitochondrial (Pccb) ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the accurate detection of mouse Propionyl-CoA carboxylase beta chain, mitochondrial (Pccb) levels in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay shows reactivity with mouse samples and is intended for research use only.

The kit delivers a sensitivity of 0.164ng/mL with a detection range of 0.312-20ng/ml. Precision is supported by an intra-assay CV of 6.8% and an inter-assay CV of 8.6%. Recovery values average 99% (range 93-105%) for serum and 101% (range 95-107%) for plasma. Linearity has been demonstrated for serum, EDTA plasma and heparin plasma across dilution factors of 1:2, 1:4, 1:8 and 1:16. The antibody pair is specific for natural and recombinant mouse Propionyl-CoA carboxylase beta chain, mitochondrial.

PCCB is a key enzyme involved in the breakdown of branched-chain amino acids and fatty acids, playing a critical role in energy production and metabolism. The protein localises to the mitochondrion matrix, belongs to the AccD/PCCB family and is listed under UniProt Q99MN9 with a molecular weight of 58,409 Da. Dysregulation of PCCB has been linked to various metabolic disorders, making it a useful biomarker for studying these conditions and potential therapeutic interventions. Synonyms include Propanoyl-CoA:carbon dioxide ligase subunit beta and PCCase subunit beta.