Mouse Proopiomelanocortin (POMC) ELISA Kit

Quantitative measurement of proopiomelanocortin (POMC) in mouse research samples with this 96-assay colorimetric kit from Assay Genie. POMC is a prohormone precursor that is cleaved in a tissue-specific manner to generate multiple bioactive peptides, including adrenocorticotropic hormone (ACTH), α-melanocyte-stimulating hormone (α-MSH), β-MSH, γ-MSH, and β-endorphin, which together govern appetite regulation, energy balance, HPA-axis stress responses, and skin pigmentation.

Investigators studying obesity, leptin and melanocortin signaling, hypothalamic control of feeding, and glucocorticoid biology use this assay to quantify circulating POMC-derived peptides in mouse specimens, particularly in serum specimens as well as heparin- and EDTA-treated plasma from experimental cohorts. Common applications include characterizing POMC neuron activity in diet-induced obesity models, screening melanocortin receptor agonists and antagonists, profiling neuroendocrine responses to stress, and tracking α-MSH and ACTH release under varied experimental conditions.

The microplate-based protocol yields an absorbance signal proportional to POMC concentration in each well, and the kit provides reagents to support standard ELISA workflows for in vitro research use only. Capture antibodies are directed against the mouse POMC sequence, ensuring reactivity with full-length prohormone and processed peptides of interest.