Mouse Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) ELISA Kit

Quantitative measurement of mouse prolyl-4-hydroxylase alpha-1 (P4HA1) supports research into collagen biosynthesis, fibrosis progression, and extracellular matrix remodeling. This colorimetric ELISA kit from Assay Genie targets the catalytic component of prolyl-4-hydroxylase, an endoplasmic reticulum-resident enzyme that converts proline residues to 4-hydroxyproline during collagen propeptide maturation. It is a relevant tool for studies of wound healing, tissue scarring, hypoxic signaling, and connective tissue disease models in murine systems.

Validated for mouse reactivity, the kit works with tissue homogenates and accommodates crude preparations without extensive pre-purification. Each box contains reagents sufficient for 96 assays in a microplate format, with a colorimetric readout compatible with standard plate readers. Sample volumes are modest, and the workflow is intended for in vitro research use only, making the kit accessible for laboratories tracking P4HA1 levels across experimental conditions, genetic backgrounds, or disease-model comparisons.

Reagents should be stored refrigerated, and repeated freeze-thaw cycles should be avoided to preserve enzymatic activity and antibody binding. The kit's components are configured for single-use aliquoting to maintain stability across multi-day experimental runs.