Mouse Proliferating cell nuclear antigen (Pcna) ELISA Kit

A sandwich ELISA from Assay Genie, this kit is designed for the quantitative detection of mouse Proliferating Cell Nuclear Antigen (PCNA), a key protein involved in DNA replication and repair that also serves as a marker for cell proliferation. The assay supports research into cell growth and division, cancer biology, and related disease mechanisms using mouse models.

Validated for use with serum, plasma, cell culture supernatants, tissue homogenates and other biological fluids, the kit is specific for natural and recombinant mouse PCNA (UniProt P17918). The target is also listed under the synonyms Cyclin and PCNA. Detection is by ELISA with colorimetric readout. The kit is supplied for research use only.

Performance characteristics include a detection range of 0.156–10 ng/mL and a sensitivity of 0.084 ng/mL. Reported precision is an intra-assay CV of 6.8% and an inter-assay CV of 8.6%. Linearity has been demonstrated across serial dilutions in serum (90–118%), EDTA plasma (90–114%) and heparin plasma (88–118%). Recovery averages 99% for serum (range 93–105%) and 101% for plasma (range 95–107%).