Mouse Prolactin PharmaGenie ELISA Kit (SBRS1510)

Quantitative measurement of mouse prolactin (mPRL), a peptide hormone secreted by the anterior pituitary gland, is supported by the Assay Genie Mouse Prolactin PharmaGenie ELISA kit, which accepts research samples derived from conditioned cell culture media, plasma, or serum. The assay employs a matched-antibody-pair configuration with chromogenic signal generation, producing absorbance values proportional to mPRL concentration across a generated standard curve. Mouse prolactin plays central roles in mammary gland development, lactogenesis, reproductive function, and immunomodulation, supporting applications in endocrinology, lactation biology, reproduction research, and pituitary-function studies.

Each kit is configured for up to 96 assays and includes pre-coated microplate wells, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant mouse prolactin standards, assay buffer, wash buffer, substrate solution, and stop solution. The workflow involves sequential incubation of samples and standards, washing, detection-antibody binding, and chromogenic readout on a standard microplate reader. Reconstituted reagents should be stored refrigerated and protected from light, and the microplate should remain sealed with desiccant when not in use to preserve antibody activity across the kit's shelf life.