Mouse Prokineticin 2 (PK2) ELISA Kit

Prokineticin 2 (PK2) is a secreted cysteine-rich signaling protein that acts through G-protein coupled receptors PROKR1 and PROKR2, regulating circadian rhythms within the suprachiasmatic nucleus, neurogenesis, angiogenesis, and gastrointestinal motility. Assay Genie provides this quantitative immunoassay to help researchers measure endogenous PK2 levels in rodent biological samples.

Kit components include a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash concentrate, TMB substrate, and stop solution. A typical protocol loads 50-100 uL of sample per well, followed by incubation, washing, and signal development steps. Store unopened reagents refrigerated at 4 degrees C; protect the TMB vial from light and minimize freeze-thaw cycles of reconstituted standards.

Common research applications include inflammatory bowel disease models, neuropathic pain studies, circadian disruption, reproductive biology, and tumor angiogenesis investigations. Intended for research use only, not for diagnostic procedures.