Mouse Proinsulin ELISA Kit

Assay Genie offers a sandwich ELISA kit for the quantitative measurement of proinsulin in mouse samples. The double-antibody format is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, supporting research into diabetes, metabolic disorders, and pancreatic beta-cell function, where proinsulin serves as a key precursor to insulin and a biomarker of blood glucose regulation.

Reported performance includes a sensitivity of 0.75 pg/mL and a working range of 1.25–80 pg/mL. Linearity at 1:2, 1:4 and 1:8 dilutions (n = 5) is given as 88–105%, 89–103% and 86–94% for serum; 82–96%, 86–99% and 84–99% for EDTA plasma; and 80–100%, 80–99% and 80–95% for heparin plasma. Recovery ranges are 88–105% (average 98%) for serum, 87–102% (average 92%) for EDTA plasma, and 85–104% (average 97%) for heparin plasma. The kit is stored at 2–8°C for 12 months, and a third-party listing describes the format as solid phase.

Sample preparation guidance covers clotting and centrifugation at 1000 × g for 20 minutes for serum, anticoagulant-tube collection with centrifugation at 1000 × g for 15 minutes at 2–8°C for plasma, homogenisation in PBS with protease inhibitors for tissue, centrifugation at 2500 rpm for 5 minutes for cell culture supernatant, and lysis-buffer treatment with protease inhibitors for cell lysates. The assay follows six steps: reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin (SABC) binding at 37°C for 30 minutes, TMB colour development in the dark for 10–20 minutes, and absorbance reading at 450 nm after stop solution. Proinsulin is also listed under the synonym PI, and the research area is identified as Cell Biology.