Mouse Programmed cell death protein 1 (Pdcd1) ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the quantitative detection of mouse Programmed cell death protein 1 (Pdcd1), also known by the synonyms CD279, Protein PD-1 and Pd1. Pdcd1 is described as an inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance, inhibiting T-cell effector functions in an antigen-specific manner upon ligand binding, with cancer listed as a research area.

Quoted performance characteristics include a detection range of 0.156-10ng/ml and a sensitivity of 0.078ng/mL. Intra-assay precision is given as 6.4% CV and inter-assay precision as 8.5% CV. Linearity in serum (N=5) is reported as 87-97% at 1:2, 89-101% at 1:4, 93-105% at 1:8 and 108-117% at 1:16; in EDTA plasma (N=5) as 97-109% at 1:2, 83-92% at 1:4, 100-109% at 1:8 and 87-97% at 1:16; in heparin plasma (N=5) as 90-99% at 1:2, 99-108% at 1:4, 102-112% at 1:8 and 84-94% at 1:16. Reported recovery averages 99% for serum (range 93-105%) and 101% for plasma (range 95-107%).

The kit is reactive with mouse samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and shows specificity for natural and recombinant mouse Programmed cell death protein 1. The target is reported as a monomer that functions as a single-pass type I membrane protein with a molecular weight of 50-55kDa (UniProt Q02242). For research use only.