Mouse Proenkephalin (PENK) ELISA Kit

From Assay Genie, this quantitative enzyme immunoassay reagent enables measurement of endogenous mouse proenkephalin (PENK), a polypeptide precursor processed into the enkephalin family of opioid neuropeptides. The kit supports research where proenkephalin abundance serves as an indicator of enkephalinergic activity in rodent model systems.

Application areas include investigations of chronic pain pathways, stress-axis biology, neuroinflammatory responses, reward and addiction circuitry, and neuropeptide hormone processing in mouse studies. Researchers examining analgesic mechanisms, behavioral neuroscience, or G-protein coupled receptor signaling often quantify PENK alongside other endogenous opioid peptide markers.

Each kit supplies a complete reagent set: a pre-coated strip plate, biotinylated detection antibody, lyophilized reference standard, streptavidin-HRP conjugate, concentrated wash buffer, chromogenic substrate solution, and stop reagent in sealed vial containers. The microplate layout accommodates parallel processing of calibration standards and experimental unknowns, with reagents for a single full-plate run.

Intended for research use only in in vitro laboratory workflows, applied to mouse-derived blood fractions and homogenized tissue specimens collected from experimental cohorts. Refrigerate kit components upon receipt, equilibrate to room temperature before initiating the protocol, aliquot reconstituted standards to minimize freeze-thaw cycles, and protect the chromogenic substrate from light exposure.