Mouse Pro-epidermal growth factor (Egf) ELISA Kit

Built around the mouse Pro-epidermal growth factor target, this Sandwich ELISA Kit from Assay Genie is designed for the accurate detection of EGF levels in mouse serum, plasma, and cell culture supernatants. The colorimetric assay is specific for natural and recombinant mouse Pro-epidermal growth factor and is supplied for research use only.

Performance is reported as a detection range of 15.6-1000pg/mL with a sensitivity of 7.8pg/mL. Precision figures are an intra-assay CV of 8.1% and an inter-assay CV of 10.2%. Linearity has been evaluated across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16, with recovery averaging 92% for serum (range 86-98%) and 94% for plasma (range 88-100%). Validated sample types include tissue homogenates, cell culture supernates, and other biological fluids, with the listed research area being cancer.

EGF is a key growth factor that regulates cell growth, proliferation, and differentiation and is involved in wound healing, tissue repair, and development. The protein is annotated (UniProt P01132) as a single-pass type I membrane protein that interacts with EGFR to promote its dimerization, and additionally interacts with RHBDF1 and RHBDF2, which can regulate its retention and degradation in the endoplasmic reticulum.