Mouse PRGN (progranulin) ELISA Kit (MOES01756)

Mouse progranulin (PGRN) is a multi-domain secreted glycoprotein encoded by the GRN gene, and Assay Genie's Mouse PRGN (progranulin) immunoassay supports quantitative measurement of this protein in research specimens. In mouse models, PGRN haploinsufficiency recapitulates aspects of frontotemporal lobar degeneration, while altered expression modulates wound healing, innate immunity, and metabolic pathways, making reliable quantification central to GRN-related research programs.

The assay uses a matched-pair antibody approach in which pre-coated capture antibodies on a 96-assay microplate bind mouse PGRN from standards or samples. Bound analyte is detected with a biotinylated reporter antibody, streptavidin-conjugated enzyme, and a chromogenic substrate read on a standard plate reader. The kit includes recombinant mouse progranulin calibrators for standard curve generation, along with assay buffer, wash concentrate, and detection reagents needed to complete the run.

Validated specimen types include mouse serum or plasma samples, as well as tissue homogenates prepared from mouse sources. For optimal performance, the kit should be refrigerated upon receipt and components used before the printed expiration date. Researchers should allow all reagents to equilibrate to room temperature before use and avoid repeated freeze-thaw cycles of working aliquots. The format provides flexibility for both small pilot studies and larger screening campaigns targeting PGRN as a biomarker of lysosomal dysfunction or neurodegeneration.