Mouse Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit

PAPPA (Pregnancy Associated Plasma Protein A) is a zinc-binding metalloproteinase secreted by placental trophoblasts, and the Assay Genie Mouse Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit provides quantitative measurement of this analyte in mouse-derived specimens for reproductive biology, pregnancy complication modeling, and cardiovascular research. The enzyme cleaves insulin-like growth factor binding proteins (IGFBP-4 and IGFBP-5), modulating local IGF bioavailability and downstream signaling relevant to fetal development, vascular remodeling, and atherosclerotic plaque instability.

Intended for laboratory research use only, the kit accommodates multiple specimen matrices including whole-blood derivatives and tissue homogenates, supporting studies in transgenic and knockout mouse strains used to investigate preeclampsia, intrauterine growth restriction, and acute coronary syndromes. Each kit supplies reagents sufficient for 96 assays in a microplate format, with assay buffers, standard, and detection antibody components for generating a standard curve across a defined concentration range.

Application areas include first- and second-trimester equivalent pregnancy phenotyping in murine models, biomarker validation for trophoblast function, longitudinal sampling from pregnant dams, and investigation of PAPPA as a marker of plaque vulnerability in vascular biology studies. Researchers performing immunohistochemistry, Western blot validation, or PCR confirmation of Pappa transcript expression often pair this quantitative assay with parallel molecular readouts to triangulate protein abundance against gene-level changes.

Store reagents refrigerated at 4 °C unless otherwise indicated on the component vial labels, and avoid repeated freeze-thaw cycles of prepared standards to preserve binding activity. Each kit ships with a lot-specific certificate of analysis and a protocol insert detailing recommended dilutions for liquid matrices commonly encountered in mouse phenotyping workflows.