Mouse Polyubiquitin-B (Ubb) ELISA Kit

A Sandwich ELISA from Assay Genie is specifically designed for the precise detection of Mouse Polyubiquitin-B (Ubb) levels in mouse serum, plasma, and cell culture supernatants. The kit delivers high sensitivity and specificity, supporting accurate and consistent results across a variety of research applications. Polyubiquitin B is a key component of the ubiquitin-proteasome system, with important roles in protein degradation and turnover; changes in UBB levels have been linked to various diseases, making the protein a valuable biomarker for studying these conditions and potential therapeutic interventions.

Quantitative performance includes a detection range of 0.312-20ng/mL, a sensitivity of 0.183ng/mL, an Intra CV of 6.4%, and an Inter CV of 8.4%. Linearity values for serum (N=5) are 105-115% at 1:2, 96-106% at 1:4, 116-126% at 1:8, and 98-106% at 1:16; for EDTA plasma (N=5) they are 106-116%, 82-92%, 104-113%, and 90-99%; and for heparin plasma (N=5) they are 108-117%, 100-112%, 101-110%, and 91-99%. Average recovery is 105% (range 99-111%) for serum and 107% (range 101-113%) for plasma.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with specificity for natural and recombinant mouse Polyubiquitin-B. The indicated research area is Neurosciences, with subcellular localisation in the cytoplasm and nucleus. UniProt reference is P0CG49. The kit is intended for research use only.