Mouse Podocin (Nphs2) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative detection of Podocin (NPHS2) in mouse samples. The assay targets the protein known by the synonyms Podocin and Nphs2 and is for research use only. The manufacturer states a detection range of 0.312–20 ng/mL with a sensitivity of 0.087 ng/mL, with specificity for natural and recombinant mouse Podocin. Acceptable sample types include serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids, and absorbance is read at 450 nm on a standard microplate reader.

The analyte is a single-pass membrane protein studied within the Signal Transduction research area, functioning as a likely linker between the plasma membrane and the cytoskeleton and contributing to the regulation of glomerular permeability. It interacts with nephrin/NPHS1, KIRRL1, CD2AP and DDN, while defects in NPHS2 are described as the cause of nephrotic syndrome type 2. The associated UniProt entry is Q91X05 and the molecular weight is listed as 42,337 Da. Supplied components include a dismountable ELISA microplate, lyophilised standard, sample and assay diluents, detection reagents A and B, wash buffer, substrate, stop solution and plate sealers; reference reagents are stored at -20°C while wash buffer, substrate and stop solution are stored at 4°C. The manufacturer positions the kit as an essential tool for researchers studying kidney disorders and seeking to advance understanding of Podocin's role in renal health.