Mouse Platelet-activating factor acetylhydrolase IB subunit gamma (Pafah1b3) ELISA Kit

From Assay Genie, this Sandwich ELISA kit enables colorimetric detection of mouse Platelet-activating factor acetylhydrolase IB subunit gamma (Pafah1b3) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. It is specific for natural and recombinant mouse Pafah1b3 and is intended for research use only.

According to the manufacturer's specifications, the assay has a detection range of 0.312-20ng/mL and a sensitivity of 0.19ng/mL. Pafah1b3 is a catalytic subunit of cytosolic PAF-AH IB that inactivates platelet-activating factor by removing the acetyl group at the sn-2 position and plays an important role during brain development. It belongs to the 'GDSL' lipolytic enzyme family, is localised to the cytoplasm, and has a reported molecular weight of 25,853 Da (UniProt Q61205; NCBI Gene ID 18476). Among its listed synonyms are PAFAH subunit gamma, PAF-AH 29 kDa subunit, PAF-AH subunit gamma, and Pafahg.

The supplied reagents include an ELISA microplate, lyophilised standard, sample and assay diluents, detection reagents, wash buffer, substrate, stop solution, and a plate sealer. Reagents are stored at -20°C, with specified components held at 4°C. The kit is suited to cardiovascular research, and additional buffer information lists shipping at +4°C.