Mouse Plasmin-Antiplasmin Complex ELISA Kit

From Assay Genie, this sandwich ELISA with double-antibody detection is designed for the quantitative measurement of plasmin-antiplasmin complex (PAP) in mouse samples. The kit supports research into fibrinolysis and the regulation of blood clot breakdown, and is relevant to studies of clotting disorders and cardiovascular disease, where dysregulation of this complex serves as a biomarker.

The assay has a sensitivity of 18.75 pg/mL and a working range of 31.25–2000 pg/mL. The solid-phase format accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is stored at 2–8°C for 12 months. Detection is colorimetric, using TMB substrate with absorbance read at 450 nm.

Linearity has been validated at dilutions of 1:2, 1:4 and 1:8, with serum (n=5) at 83–97%, 87–98% and 83–92%, EDTA plasma (n=5) at 92–100%, 88–100% and 83–96%, and heparin plasma (n=5) at 88–102%, 95–100% and 82–94%. Recovery ranges are 85–104% (serum, 92% average), 85–105% (EDTA plasma, 95% average) and 85–102% (heparin plasma, 97% average). The kit is supplied with a pre-coated dismountable microplate, lyophilized standard, biotin-labeled detection antibody (100X concentrate), HRP-streptavidin conjugate (SABC, 100X concentrate), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X concentrate), plate sealers, and a technical manual.