Mouse PKB/Protein Kinase B ELISA Kit

From Assay Genie, this colorimetric Sandwich ELISA kit is designed for the quantitative measurement of mouse Protein Kinase B (PKB), also known as Akt, using a double-antibody format. It offers a sensitivity of 0.469 ng/mL and a working range of 0.781-50 ng/mL, and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Protein Kinase B is a key enzyme in the PI3K/Akt signaling pathway, which regulates cellular processes including growth, survival, and metabolism. Dysregulation of PKB activity has been associated with cancer, diabetes, and cardiovascular disorders, making it a research target of therapeutic interest. The kit is designed to support research applications investigating PKB signaling pathways and potential treatments for related diseases.

The assay protocol includes reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, TMB substrate color development in the dark for 10–20 minutes, and absorbance measurement at 450 nm. Reported recovery averages are 93% for serum, 95% for EDTA plasma, and 95% for heparin plasma, with linearity across dilutions of 1:2, 1:4, and 1:8 ranging from 81-105%. Reagents are stored at 2-8°C for 12 months.