Mouse PKB (Protein Kinase B) ELISA Kit (MOES01412)

This absorbance-based microplate assay from Assay Genie quantifies Protein Kinase B (Akt) in murine samples. PKB/Akt sits at the heart of PI3K-AKT-mTOR signaling and governs cellular processes including glucose metabolism, cell survival, proliferation, and growth factor responses, making accurate measurement essential for diabetes, oncology, and pathway-focused studies.

Each kit contains pre-coated strip plates, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, stop solution, and standardized reagents sufficient for 96 determinations. The assay accepts tissue homogenates, cell lysates, and supernatant fractions from mouse-derived sources. Researchers commonly apply this kit to monitor insulin-stimulated Akt activation in muscle or liver lysates, track pathway activity in tumor xenograft models, and compare baseline versus treated samples in drug screening campaigns.

Bring all reagents to room temperature before use and avoid repeated freeze-thaw cycles of standard and detection antibody aliquots. The complete protocol, plate layout recommendations, and performance characteristics ship with each kit. For research use only.