Mouse Piezo-type mechanosensitive ion channel component 1 (Piezo1) ELISA Kit

Assay Genie provides a Sandwich ELISA Kit for the quantitative measurement of mouse Piezo-type mechanosensitive ion channel component 1 (Piezo1), a mechanosensitive non-specific cation channel that converts mechanical stimuli into cellular signals and is implicated in touch sensation, vascular development, and erythrocyte volume regulation. The kit is intended for research use only and is suitable for studies in cardiovascular health, sensory perception, and cellular physiology.

Validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, the assay delivers a detection range of 78-5000pg/mL with a sensitivity of 40.4pg/mL. Reproducibility is supported by an intra-assay CV of 3.5% and an inter-assay CV of 6.5%. Dilutional linearity has been verified in serum (88-101% at 1:2, 107-119% at 1:4, 85-95% at 1:8, 82-91% at 1:16), EDTA plasma (105-117%, 106-115%, 80-90%, 103-113%) and heparin plasma (109-118%, 102-112%, 101-111%, 107-117%). Spike-and-recovery ranges are 83-95% (average 89%) for serum and 85-97% (average 91%) for plasma. The kit specifically detects natural and recombinant mouse Piezo1.

The target protein (UniProt E2JF22; 292,002 Da) is a multi-pass membrane protein localising to the endoplasmic reticulum membrane, endoplasmic reticulum-Golgi intermediate compartment membrane, cell membrane, and lamellipodium membrane. Functionally, PIEZO1 acts as a pore-forming subunit of a mechanosensitive non-specific cation channel, generates currents sensitive to ruthenium red and gadolinium, contributes to epithelial cell adhesion via integrin and R-Ras signalling, acts as a shear-stress sensor in endothelial cells, and supports blood vessel formation and vascular structure in both development and adult physiology. Detection is performed using a colorimetric method.