Mouse Phospholipase A2, Calcium Independent (iPLA2) ELISA Kit

The Mouse Phospholipase A2, Calcium Independent (iPLA2) ELISA Kit from Assay Genie enables quantitative measurement of iPLA2β (PLA2G6) protein levels in murine research samples. iPLA2 is a cytosolic enzyme that catalyzes hydrolysis of the sn-2 acyl chain of glycerophospholipids, liberating free fatty acids such as arachidonic acid and generating lysophospholipid messengers that drive downstream eicosanoid signaling and inflammatory lipid cascades.

This assay supports research into mitochondrial membrane homeostasis, oxidative lipid damage, and neurodegenerative pathways, since loss-of-function mutations in PLA2G6 have been linked to parkinsonian disorders and dystonia. Investigators profiling ischemic injury, ferroptosis, or immune activation in knockout and transgenic mouse models commonly track iPLA2 expression across brain, liver, and lymphoid tissues.

Each kit contains a pre-coated microplate, biotinylated detection antibody, recombinant standards, streptavidin-HRP conjugate, wash buffer, substrate, and stop solution. Upon receipt, store unopened components refrigerated at 4 degrees C; reconstituted standards should be aliquoted and kept frozen to limit freeze-thaw cycles, while the working detection antibody remains stable in the refrigerator for short-term use. The kit is intended for research use only and is not validated for diagnostic or therapeutic applications.