Mouse Phosphoinositide Dependent Protein Kinase 1 (PDPK1) ELISA Kit

A serine/threonine kinase central to PI3K signalling, phosphoinositide-dependent protein kinase 1 (PDPK1) phosphorylates Akt at Thr308 and is quantified in murine samples by this colourimetric immunoassay from Assay Genie. The assay measures endogenous PDPK1 protein in cell lysates and tissue homogenates, fitting into research programmes that probe insulin signalling, glucose homeostasis, and tumour-cell proliferation. Its 96-assay microplate layout supports standard bench incubation and washing workflows, scaling from small pilot studies to larger cohort screens in metabolic-disease and oncology laboratories. Researchers commonly pair PDPK1 quantitation with downstream Akt phospho-status readouts at Thr308 and Ser473 when evaluating pathway engagement in response to PI3K or Akt small-molecule inhibitors, or when characterising transgenic and knockout mouse models. The kit is configured for research use only and complements Western blot and immunoprecipitation workflows that dissect AGC-family kinase regulation across mouse tissue and cell systems.