Mouse Phosphoglycerate kinase 1 (Pgk1) ELISA Kit

Designed for the precise measurement of Phosphoglycerate kinase 1 (PGK1) levels, this Sandwich ELISA Kit from Assay Genie is intended for the quantitative detection of mouse PGK1 in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit reacts with mouse samples and detects the natural and recombinant form of the target.

Performance characteristics include a detection range of 78-5000pg/mL and a sensitivity of 39.6pg/mL. Reproducibility figures are reported as an intra-assay CV of 6.8% and an inter-assay CV of 8.6%. Linearity has been evaluated in serum, EDTA plasma and heparin plasma across dilutions from 1:2 to 1:16 (N=5), with average recovery of 89% for serum (range 83-95%) and 91% for plasma (range 85-97%).

PGK1 functions as a glycolytic enzyme and is reported to act as a polymerase alpha cofactor protein (primer recognition protein), with a possible additional role in sperm motility. The protein is a monomer located in the cytoplasm (UniProt P09411; NCBI Gene ID 18655), with a stated molecular weight of 44,550 Da. For research use only.