Mouse Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ELISA Kit

The cytosolic enzyme phosphoenolpyruvate carboxykinase (PCK1) catalyzes the rate-limiting step of gluconeogenesis, converting oxaloacetate into phosphoenolpyruvate with GTP consumption. Mouse PCK1 protein abundance in liver, kidney, and adipose tissue is a key biomarker in studies of fasting physiology, insulin resistance, type 2 diabetes, and hepatic metabolic adaptation. The Assay Genie quantitative immunoassay targets the soluble cytosolic PCK1 isoform specifically in mouse samples and is supplied as a complete kit sized for 96 individual determinations on a standard microplate.

Intended for use with tissue homogenates prepared from fresh or snap-frozen mouse organs, the kit includes reagents for measuring PCK1 across cytosolic lysates. The colorimetric readout enables straightforward absorbance measurement using a standard plate reader. Researchers apply this kit to expression profiling of gluconeogenic tissues, time-course studies of fasting or refeeding, comparison of PCK1 regulation between wild-type and transgenic mouse strains, and investigations linking hepatic PCK1 activity to tumor metabolic phenotypes. Each pack contains all assay reagents pre-configured for the 96-assay run size, supporting both small pilot experiments and larger metabolic disease cohorts.