Mouse Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit

PDE3A is a cGMP-inhibited cyclic nucleotide phosphodiesterase central to vascular smooth muscle regulation, platelet aggregation, and cardiac contractility, and is the molecular target of clinically used agents such as milrinone and cilostazol. The Mouse Phosphodiesterase 3A (PDE3A) ELISA Kit from Assay Genie provides quantitative measurement of this enzyme in murine samples, supporting cardiovascular, metabolic, and pharmacological research programs.

Validated for tissue homogenates and configured for 96 assays, the kit uses a colorimetric microplate readout compatible with standard laboratory plate readers. Researchers can apply the assay to compare PDE3A expression across treatment groups, transgenic models, or disease states such as hypertension, heart failure, and metabolic syndrome. Each kit includes a pre-coated plate, detection reagents, reference standards, and assay buffers sufficient for the indicated number of determinations.

The PDE3A target is relevant to studies of insulin signaling in adipocytes and hepatocytes, vascular remodeling after injury, and contractile responses in cardiac tissue. Sample preparation should follow standard protocols for protein extraction from fresh or frozen tissue. Intended for research use only. Review the enclosed product insert for detailed protocol, sample dilution guidance, and performance characteristics before initiating the assay.