Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (Pik3ca) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative detection of mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (Pik3ca), and is intended for research use only. The assay is reactive with mouse samples and supports research in immunology, where PIK3CA is studied for its role in the PI3K/AKT signaling pathway regulating cell growth, proliferation, and survival.

Reported quantitative performance includes a detection range of 0.78-50 ng/mL and a sensitivity of 0.39 ng/mL, with an intra-assay CV of 6.2% and an inter-assay CV of 11.3%. Linearity in serum spans 84-120% across 1:2 to 1:16 dilutions (N=5); EDTA plasma spans 99-120% and heparin plasma spans 97-123% over the same dilutions. Recovery averages 89% (range 83-95%) for serum and 91% (range 85-97%) for plasma. The kit specifically detects natural and recombinant mouse Pik3ca.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. Listed synonyms for the target are Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PtdIns-3-kinase subunit p110-alpha, and PI3-kinase subunit alpha. Storage is at -20°C; shipping is on cold packs at +4°C.