Mouse Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (Pten) ELISA Kit

Quantify mouse PTEN tumor suppressor protein with this microplate-format immunoassay from Assay Genie. PTEN functions as a 3-phosphatase that dephosphorylates phosphatidylinositol-3,4,5-trisphosphate, directly opposing PI3K signaling and thereby restraining AKT-driven cell growth, survival, and metabolic pathways.

It is one of the most frequently inactivated tumor suppressors in human cancer and is widely studied in murine models of oncogenesis, insulin resistance, and neurodevelopment. The assay uses matched capture and detection antibodies bound to a pre-coated plate, with an HRP-conjugated streptavidin step and TMB substrate yielding an absorbance readout on a standard microplate reader.

Validated reactivity covers serum, EDTA-plasma, and clarified tissue homogenate specimens harvested from laboratory mice, supporting research use only applications in pathway interrogation, biomarker discovery, and characterization of conditional knockouts and transgenic lines.

Sufficient reagents are provided in individual vials for one full plate, and the protocol fits a standard working-day schedule. Antibody components should be kept refrigerated; aliquoting upon arrival minimizes freeze-thaw loss of activity, and the reconstituted standard should be used promptly to maintain calibration integrity.