Mouse Phex  (Phosphate-regulating neutral endopeptidase PHEX) ELISA Kit (MOFI01362)

Built for quantitative immunology research in mouse models, this Assay Genie ELISA Kit measures circulating levels of Phosphate-regulating neutral endopeptidase, a membrane-bound metalloprotease encoded on the X chromosome and expressed predominantly in osteoblasts, osteocytes, and odontoblasts. PHEX regulates FGF23 processing and plays a central role in skeletal phosphate handling, making it a target of interest in studies of X-linked hypophosphatemia, vitamin D metabolism, and chronic kidney disease-mineral bone disorder.

Each kit is supplied in a microplate format with reagents sufficient for the indicated number of determinations and reference standards for generating a calibration curve. The absorbance-based readout allows direct quantitation in murine serum or plasma specimens. Best results are obtained when samples are separated promptly after collection, aliquoted to avoid repeated freeze-thaw cycles, and stored refrigerated until the time of assay.

Investigators using genetic knockouts, hypophosphatemic rickets models, or pharmacological interventions affecting phosphate transport can integrate this kit into in vivo and in vitro study designs. The product is for research use only and is not intended for clinical diagnostic applications.