Mouse PHD Finger Protein 8 (PHF8) ELISA Kit

A Jumonji C (JmjC) domain-containing histone demethylase, mouse PHD Finger Protein 8 (PHF8) acts as a transcriptional co-activator in neural progenitors and has been implicated in X-linked intellectual disability and craniofacial anomalies in human patients. The corresponding murine ortholog also participates in ribosomal RNA biogenesis and the DNA damage response pathway, making it a target of interest in neurodevelopmental, cancer, and chromatin biology studies. The Assay Genie Mouse PHF8 ELISA Kit provides a quantitative immunoassay platform configured to measure endogenous PHF8 concentrations in mouse-derived sample preparations.

Researchers working on epigenetic regulation can apply this 96-assay kit to mouse tissue homogenates and related sample preparations to track endogenous PHF8 levels across different developmental stages, brain regions, or disease-model backgrounds. The workflow supports several in vitro research applications, including validation of CRISPR knockout and knockdown lines, side-by-side comparison of wild-type versus mutant expression, and confirmation of transcriptomic changes observed in ribosome biogenesis or neuronal differentiation studies.

Each package includes a pre-coated microplate, calibration standards, detection antibody, and substrate solution, accompanied by a printed protocol. Reagents are formulated for refrigerated storage to preserve activity through the kit's shelf life, and aliquoting of working standards is recommended to limit freeze-thaw exposure of antibody reagents.