Mouse Peroxisome Proliferator Activated Receptor Alpha (PPARa) ELISA Kit

Quantitative measurement of peroxisome proliferator-activated receptor alpha in mouse research specimens. This colorimetric immunoassay from Assay Genie is supplied as a complete kit containing a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, TMB substrate solution, and stop reagent sufficient for 96 individual determinations. The reagents support studies of lipid metabolism, hepatic gene regulation, and nuclear receptor signaling pathways in murine models, and are validated for application with serum or plasma, tissue homogenates, cell lysates, and cell culture supernatant specimens. Components are shipped under refrigerated conditions and should be stored according to the temperatures printed on each vial label; reconstituted standards should be aliquoted and frozen at -20°C to minimize repeated freeze-thaw cycles. Each kit includes a detailed protocol with recommended dilution factors and incubation times optimized for reproducible standard curves across the assay dynamic range. Researchers investigating metabolic disorders, fatty acid oxidation pathways, atherosclerosis, hepatic steatosis, or liver-specific transcriptional regulation will find this in vitro research use only reagent appropriate for quantifying PPARα protein levels in experimental samples under controlled laboratory conditions.