Mouse Peroxiredoxin-5, mitochondrial (Prdx5) ELISA Kit

Assay Genie offers a sandwich ELISA kit designed for the precise quantification of Peroxiredoxin-5 (PRDX5), a mitochondrial peroxiredoxin protein involved in cellular protection against oxidative stress and maintenance of redox balance. The kit is reactive with mouse samples and is intended for research use only, supporting studies of apoptosis, inflammation, cell signalling, and the role of PRDX5 in conditions including cancer.

Reported performance includes a detection range of 0.78-50 ng/mL and a sensitivity of 0.452 ng/mL, with an intra-assay CV of 6.4% and inter-assay CV of 8.9%. The kit is validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and recognises natural and recombinant mouse Peroxiredoxin-5, mitochondrial. According to the manufacturer, PRDX5 functions as a thiol-specific peroxidase that catalyses the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols.

Sample preparation guidance covers serum, plasma (EDTA or heparin), urine, cerebrospinal fluid, cell culture supernatant, cell lysates and tissue homogenates, with linearity and recovery data provided for serum, EDTA plasma and heparin plasma dilutions. The kit is designated for the Cancer research area.